C. elegans, but not as you know it

Automated imaging for health, not death

The Challenge

Manual C. elegans assays cannot cope with higher demands for throughput efficiency and data reproducibility. Our unique technology can transform your science with:

A robust workflow for high data reproducibility
Non-invasive and exhaustive data acquisition
Versatile data processing to reveal subtle intervention effects

Measuring ageing and toxicity in C. elegans with manual lifespan assays is well-established in academic research, but their lack of throughput efficiency and data reproducibility are significant barriers to their adoption by industry. Manual C. elegans lifespans assays:

Are very time-consuming, require skill and are subject to operator variation.
Use only a few widely-spaced time points.Disrupt the worms and their environment at each manipulation.
Give single data points (live/dead) not continuous (e. g. speed) data.

The Magnitude Biosciences Solution: Automated C. elegans Imaging

Our technology supports a robust and fast assay workflow. We can achieve high data reproducibility and sensitivity, thanks to:

Manual preparation consistency, with a defined medium for bacterial growth and strict schedule for plate pouring, bacterial and worm culture, as well as automated imaging for standardised data acquisition and processing.
Non-invasive data acquisition: no mechanical disruption, no abrupt changes in lighting or temperature.
Exhaustive data acquisition: multiple culture plates with up to 30 worms on each, individual worm tracking images taken every 0.8 seconds for 160 seconds, repeated every 5 minutes up to 10 days.

Lifespan Vs Healthspan

Traditional lifespan manual C. elegans approach

Magnitude Biosciences’ automated Healthspan approach and technology

Manual approach runs the risk of operator variability, reducing reproducibility

Our automated mobility tracking technology standardises the environment, thus standardising the data acquisition and causing no disruption to the worms.

Visual scoring of live/dead worms is time-consuming

Automated approach is much faster with more consistent results, minimising the time taken to do an experiment and how many experiments are needed

The invasiveness of a manual approach means the results are more variable and less objective

Our automated approach is non-invasive, yielding more objective results

Lifespan manual approach only produces one endpoint

Healthspan approach produces multiple endpoints, and the standardisation means this data is also more reliable

Manual approach is less efficient

Automation enables a much higher throughput of experiments

Lifespan

Traditional lifespan manual C. elegans approach

Health

Magnitude Biosciences’ automated Healthspan approach and technology

Lifespan Vs

Healthspan

Traditional lifespan manual C. elegans approach

Magnitude Biosciences’ automated Healthspan approach and technology

Manual approach runs the risk of operator variability, reducing reproducibility

Our automated mobility tracking technology standardises the environment, thus standardising the data acquisition and causing no disruption to the worms.

Visual scoring of live/dead worms is time-consuming

Automated approach is much faster with more consistent results, minimising the time taken to do an experiment and how many experiments are needed

The invasiveness of a manual approach means the results are more variable and less objective

Our automated approach is non-invasive, yielding more objective results

Lifespan manual approach only produces one endpoint

Healthspan approach produces multiple endpoints, and the standardisation means this data is also more reliable

Manual approach is less efficient

Automation enables a much higher throughput of experiments

1
Manual approach runs the risk of operator variability, reducing reproducibility
2
Our automated mobility tracking technology standardises the environment, thus standardising the data acquisition and causing no disruption to the worms.
Visual scoring of live/dead worms is time-consuming
3
Automated approach is much faster with more consistent results, minimising the time taken to do an experiment and how many experiments are needed
The invasiveness of a manual approach means the results are more variable and less objective
4
Our automated approach is non-invasive, yielding more objective results
Lifespan manual approach only produces one endpoint
5
Healthspan approach produces multiple endpoints, and the standardisation means this data is also more reliable
Manual approach is less efficient
6
Automation enables a much higher throughput of experiments

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