Automated imaging for health, not death

Manual C. elegans assays cannot cope with higher demands for throughput efficiency and data reproducibility. Our technology can transform your science with:

  • A robust workflow for high data reproducibility 
  • Non-invasive and exhaustive data acquisition 
  • Versatile data processing to reveal subtle intervention effects

Traditional C. elegans assays

Measuring ageing and toxicity in C. elegans with manual lifespan assays is well-established in academic research, but their lack of throughput efficiency and data reproducibility are significant barriers to their adoption by industry. Manual C. elegans lifespans assays: 

  • are very time-consuming, require skill and are subject to operator variation
  • use only a few widely-spaced timepoints
  • disrupt the worms and their environment at each manipulation
  • give single data points (live/dead) not continuous (e. g. speed) data

 

The Magnitude Biosciences Solution: Automated C. elegans Imaging 

Our technology supports a robust and fast assay workflow. We can achieve high data reproducibility and sensitivity, thanks to:

  • manual preparation consistency, with a defined medium for bacterial growth and strict schedule for plate pouring, bacterial and worm culture, as well as automated imaging for standardised data acquisition and processing. 
  • non-invasive data acquisition: no mechanical disruption, no abrupt changes in lighting or temperature.
  • exhaustive data acquisition: multiple culture plates with up to 50 worms on each, individual worm tracking images taken every 0.8 seconds for 160 seconds, repeated every 5 minutes up to 10 days

 

Analysis and Data

 

Bacterial folate synthesis inhibition by SMX, showing dose-dependent decline in moving worms over 14 days, with many more data points than manual lifespan assays (Virk et al., 2012; 400 worms per condition).

 

 

Exploration: Individual worm tracks over a set time period, showing control worms (left) explore more of their environment than test worms (right) (superimposed tracks of 20 worms).

Colour-coded segmentation of the worm population by speed, giving detailed age-related decline in mobility compared to average speed (400 worms per condition).

Mobility decline over 6 days of the human amyloid-beta1-42 muscle-expressing GMC101 strain, compared to the AM134 control strain (180 worms per condition, top: decline in the fraction of moving worms over time, bottom: mean moving hours for each worm over the whole time period).

 

Chemotaxis: Tracking of individual worms position over time from release site (green circle) to lawn site (yellow circle), to pinpoint the rapid dynamics of chemotaxis (60 worms total, collated over 4 plates of 15 worms each).

Wanna see more of our data?
Could it apply to your research interests?
Ask us for our Example Data or check out our Research Services pages!