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We believe our technology can transform your science. Here's how.

Manual assays are the traditional way of measuring ageing and toxicity in C. elegans, but they cannot cope with higher demands for throughput efficiency and data reproducibility.

 

Manual C. elegans lifespans assays:

  • are very time-consuming, require skill and are subject to operator variation 
  • use only a few widely-spaced timepoints
  • disrupt the worms and their environment at each manipulation
  • give single data points (live/dead) not continuous (e. g. speed) data

 

The Magnitude Biosciences Solution: Automated imaging for health, not death 

  • High data reproducibility: manual preparation consistency, with a defined medium for bacterial growth and strict schedule for plate pouring, bacterial and worm culture, as well as automated imaging for standardised data acquisition and processing

 

  • Non-invasive data acquisition: no mechanical disruption, no abrupt light changes, no abrupt temperature changes

 

  • Exhaustive data acquisition: 32 worm culture plates per machine with up to 50 worms per plate, individual worm tracking images taken every 0.8 seconds for 160 seconds, repeated every 5 minutes up to 10 days

 

Our technology supports a robust and fast assay workflow 

 

Week 1

Compound & Worm Preparation

  • Confidentiality
  • Solubility
  • Bacterial lawn
  • Worm growth

Week 2

Healthspan & Automation

  • Temperature/Humidity
  • Consistency
  • Peak movement
  • Decline with age

Week 3

Analysis & Data

  • Analyse variation
  • Movement distribution
  • Calculate healthspan
  • Report

Analysis and Data

Bacterial folate synthesis inhibition by SMX, showing dose-dependent decline in moving worms over 14 days, with many more data points than manual lifespan assays (Virk et al., 2012; 400 worms per condition).

 

 

Exploration: Individual worm tracks over a set time period, showing control worms (left) explore more of their environment than test worms (right) (superimposed tracks of 20 worms).

 

Colour-coded segmentation of the worm population by speed, giving detailed age-related decline in mobility compared to average speed (400 worms per condition).

Mobility decline over 6 days of the human amyloid-beta1-42 muscle-expressing GMC101 strain, compared to the AM134 control strain (180 worms per condition, top: decline in the fraction of moving worms over time, bottom: mean moving hours for each worm over the whole time period).

 

Chemotaxis: Tracking of individual worms position over time from release site (green circle) to lawn site (yellow circle), to pinpoint the rapid dynamics of chemotaxis (60 worms total, collated over 4 plates of 15 worms each).

 

Like the look of our data but not sure why we do it in worms rather than mice? Read on to learn why worms are best.

 

Can our technology help your specific field of research?

If ageing, neurodegeneration, microbiome-host interactions or toxicity are your thing, check out our Research Services page. 

And if you can’t find your assay in there, get in touch. We’re always excited to help our clients design the best custom project to answer their research question.

Magnitude Biosciences aims to serve the global field of ageing research an drug discovery. We are proud to offer a robust commercial service for all that is rapid and cost effective. We are verry happy to discuss customer needs and partnering opportunities.